Be Greater. Together. 
Appearances
Featured Characters:
- Spider-Man (Peter Parker) (Main story and flashback) (Temporarily bonds with the Venom Symbiote and bonds with the Anti-Venom Symbiote)
- Spider-Man (Miles Morales)
Supporting Characters:
- Mary Jane Watson (Temporarily bonds with the Scream Symbiote) (Leaves Daily Bugle) (Impersonates Vika) (First appearance as Scream)
- Oscorp Industries
- Norman Osborn (Main story and flashback)
- Ganke Lee
- Rio Morales
- Cooper Art Coalition
- Mister Negative (Martin Li)
- Wraith (Yuri Watanabe) (First appearance as Wraith)
Antagonists:
- Sandman (Flint Marko) (First full appearance)
- Kraven's Hunters (First appearance)
- Kraven the Hunter (Sergei Kravinoff) (First appearance; dies)
- Grizzly (Maxwell Markham) (First appearance)
- Team Alpha
- Captain Endre
- Maria
- Vidor
- Team Echo
- Captain Vika
- Hyena (Dominic)
- Mongoose (Garin)
- Team Delta
- Loris (Captain Aksana)
- Dingo (Isabella)
- Lynx (Jacob)
- Numerous unnamed hunters
- The Lizard (Curt Connors)
- Symbiote Hive (First appearance)
- Venom (Harry Osborn) (Main story and flashback) (Loses and regains the Venom Symbiote) (First appearance as Venom) (Death and resurrection; goes into a coma)
- Venom Symbiote (Temporarily separates from Harry Osborn and temporarily bonds with Spider-Man) (Apparent death)
- Scream Symbiote (First appearance) (Bonds with Mary Jane Watson) (Apparent death)
- Numerous unnamed symbiotes (Apparent death)
- Followers of the Flame (First appearance)
- The Flame (Cletus Kasady) (First appearance)
- Doug (Mentioned)
- Unnamed cultists
- Mysterio (Illusion or holographic simulation)
- Betsy Schneider (First appearance)
- Cole Wittman (First appearance)
- Mister Negative's Demons (Illusion or holographic simulation)
- Darren Shipman (First appearance)
Other Characters:
- May Parker (Only in flashback) (Deceased)
- New York Bulletin (First appearance)
- Mysterio (Quentin Beck)
- Emily-May Foundation (First appearance)
- Doctor Foster (First appearance)
- Doctor Nguyen (First appearance)
- Doctor Em Young (First appearance)
- New York City Fire Department
- Chief Clarke
- Brooklyn Visions Academy
- Midtown High
- RJ (First appearance)
- Albert Moon (First appearance)
- Cindy Moon (First appearance)
- Black Cat (Felicia Hardy)
- Aaron Davis
- Great Hunter (First appearance; dies)
- Tombstone (Lonnie Lincoln)
- Sinister Six (Only in flashback)
- Scorpion (Mac Gargan) (Death)
- Doctor Octopus (Otto Octavius)
- Rhino (Aleksei Sytsevich) (Only in recap) (appears on screen also) (Status Unknown)
- Vulture (Adrian Toomes) (Appears on screen) (Deceased)
- Electro (Maxwell Dillon) (Appears on screen) (Deceased)
- Kraven's Family
- Chameleon (Dmitri Smerdyakov) (First appearance)
- Sasha Kravinoff (Voice only)
- Nikolai Kravinoff (Mentioned)
- Anna Kravinoff (Mentioned)
- Vladimir Kravinoff (Voice only)
- Anastasia Tatiana Kravinoff (Mentioned)
- Nedrocci Tannengarden (Mentioned)
- Delilah (First appearance)
- John Jonah Jameson
- Howard (Death)
- Dima (First appearance)
- Alma Alvardo (First appearance)
- Teo Álvarez
- Spider-Man (Cat)
- Timekeeper (First appearance)
- Unnamed red and black colored symbiote (First appearance)
- Danikast
- Keemia Alvarado (First appearance)
- Tasha (First appearance)
- Earl (First appearance)
- Emily Osborn (Voice only)
- Shocker (Herman Schultz) (Appears on screen) (Deceased)
- Taskmaster (Anthony Masters) (Appears on screen) (Status unknown)
- Kingpin (Wilson Fisk) (Appears on screen) (Status unknown)
- Jeff Davis (Illusion or holographic simulation)
- Uncle Ben (Photo)
- Flash Thompson (Photo)
- Lockjaw (Statue)
- Phin Mason (Corpse, skeleton or other remains)
- Rick Mason (Corpse, skeleton or other remains)
- Wong (Behind the scenes)
- Doctor Strange (Mentioned)
- Hank Pym (Mentioned)
- Anya Corazón (Mentioned)
- Gloria Morales (Mentioned)
- Andi Benton (Mentioned)
- Lord Voldemort (Topical Reference) (Mentioned)
- Sonya Smerdyakov (Mentioned)
- Trask Industries (Mentioned)
- Rand Corporation (Mentioned)
- Trapster (Mentioned)
- Mayoress Grant (Mentioned)
- King Arthur (Mentioned)
- Farley Stillwell (Mentioned)
- Nelson and Murdock (Mentioned)
- Matt Murdock (Mentioned)
- Foggy Nelson (Mentioned)
- Jennifer Walters (Mentioned)
- Miguel O'Hara (Mentioned)
- Randy Robertson (Mentioned)
- Dr. Morgan Michaels (Named only)
- Dr. Vincent Stegron (Named only)
- Billy Connors (Photo)
- Martha Connors (Photo)
- Speed Demon (James Sanders) (Photo)
- Time-Twisters (Referenced)
- Big Wheel (Referenced)
- Hydro-Man (Referenced)
- Rocket Racer (Referenced)
- Overdrive (Referenced)
- Arcade (R. Cadenski) (Referenced)
- King Crab (Referenced)
- The Enforcer (Referenced)
- Hydra (Referenced)
- Mantis (Referenced)
- Glitz (Referenced)
- Dazzler (Referenced)
- Fantastic Four (Referenced)
- Avengers (Referenced)
- Damage Control (Referenced)
- Union Allied Construction (Named only)
- Mick (Statue)
- Crusher Hogan (Photo)
- The Underground (Mentioned)
- Roxxon (Mentioned)
- Blind Al (Alma) (First appearance)
- F1D0 "Fido" (First appearance)
- Hammerhead (Joseph Martello) (Mentioned)
- Gabriel Jones (Mentioned)
- Danny "Drummer" Bellaman (Mentioned)
- S.H.I.E.L.D. (Photo)
- Clyde Stubblefield (Mentioned)
- Charlie Parker (Mentioned)
- Florence Mills (Mentioned)
- Josephine Baker (Mentioned)
- Hazel Scott (Mentioned)
- Langston Hughes (Mentioned)
- Claude McKay (Mentioned)
- Gregor Mendel (Topical reference)
- Sable International (Mentioned)
- Silver Sable (Mentioned)
- Alias Investigations (Named only)
Locations:
- Earth-1048
- Earth
- North America
- United States of America
- New York
- New York City
- Queens
- Manhattan
- Midtown
- Times Square
- Baxter Building
- Fifth Avenue
- Union Square
- Murray Hill
- Columbus Circle
- Turtle Bay
- Hell's Kitchen
- Madison Square Garden
- Central Park South
- Rockefeller Center
- 42nd Street
- Bryant Park
- Grand Central Station
- Colexco Building
- Lexington Avenue
- 14th Street
- Flatiron District (Main story and flashback)
- Flatiron Building (Main story and flashback)
- 53rd Street
- 42nd Street
- Madison Avenue
- Upper East Side
- Harlem
- Rand Building
- The Bar with No Name
- Harlem Seafood Market
- Chinatown
- Lower East Side
- Central Park
- Greenwich Village
- Empire State University
- Upper West Side
- Tribeca
- Financial District
- Midtown
- Brooklyn
- Raft
- Coney Island
- Mysterium (First appearance)
- Ravencroft Institute (Mentioned)
- New York City
- New Jersey
- Alaska (Mentioned)
- New York
- United States of America
- Antarctica
- Europe
- Nepal (Mentioned)
- Madripoor (Named only)
- Lowtown District (Named only)
- Genosha (Named only)
- Africa (Mentioned)
- South Africa (Mentioned)
- Wakanda (Mentioned)
- North America
- Darkforce Dimension (Referenced)
- Negative Zone (Referenced)
- Astral Plane
- Symbiote Hive-Mind
- Earth
- Unidentified Location
Items:
- Spider-Men's Suits, Utility Belt, and Web-Shooters
- Spider-Man's Advanced Suit
- Anti-Venom Symbiote (First appearance)
- Scarlet Spider's Suits
- Spider-Men's 2099 Suits
- Superior Spider-Man's Suit
- Spider-Man's Anti-Ock Suit
- Arachknight's Suit
- Spider-Man's Noir Suit
- Iron Spider Armors
- Webbed Black Suit
- Classic Black Suit
- Spider-Man's Purple Reign Suit
- Forever Suit
- Spider-Man's Programmable Matter Suit
- Best There Is Suit
- Dark Ages Suit
- Absolute Carnage Suit
- King in Black Suit
- Spider-Man's Stealth Suit (Named only)
- Spider-Man's Wings
- Wand of Watoomb
- Vulture's Wings
- Shocker's Vibro-Shock Gauntlets
- Meteorite
- Talon Drones (First appearance)
- "Dog Drone"
- Lizard Serum
- Masks
- Spider-Man's Mask
- Chameleon's Mask
- Blue Mask
- Numerous other masks
- Flamethrowers
- Rhino Armor (Only in recap) (Appears on screen also)
- Doctor Octopus' Tentacles (Only in recap) (Appears on screen also)
- Prowler's Suit (Only on screen as a static image or video record)
- Devil's Breath (Only in recap)
- Goblin Formula (Mentioned)
- Captain America's Shield (Frisbee)
- NuForm (Mentioned)
Vehicles:
- Jetski
- Train
- Spider-Mobile (Mentioned)
Cast
- Yuri Lowenthal as Spider-Man/Peter Parker
- Nadji Jeter as Spider-Man/Miles Morales
- Laura Bailey as Mary Jane Watson
- Morla Gorrondona and Caitlin Hutson (both uncredited) as Scream
- Griffin Puatu as Ganke Lee
- Graham Phillips as Harry Osborn
- Mark Rolston as Norman Osborn
- Jim Pirri as Kraven the Hunter/Sergei Kravinoff and Chameleon/Dmitri Smerdyakov
- Tony Todd as Venom
- Stephen Oyoung as Mister Negative/Martin Li
- Leandro Cano as Sandman/Flint Marko
- Corey Jones as Tombstone/Alonzo Lincoln
- Mark Witten as Curtis Connors
- Erica Lindbeck as Black Cat/Felicia Hardy
- Jason Spisak as Scorpion/Mac Gargan
- Noshir Dalal as Quentin Beck and Mysterio (Illusion)
- Tara Platt as Wraith/Yuriko Watanabe
- Chad Doreck as The Flame/Cletus Kasady
- Ike Amadi as Aaron Davis
- William Salyers as Otto Octavius
- Darin De Paul as J. Jonah Jameson
- Ashly Burch as Danika Hart
- Natasha Ofili as Hailey Cooper
- Jacqueline Pinol as Rio Morales
- Russell Richardson as Jefferson Davis
- Nancy Linari as May Parker
- Nathan Fillion as Spider-Man (Cat)
- Alan Tudyk as Copycat Mascot
- Mara Junot (uncredited) as Principal Evans
- Krizia Bajos as Camila Vasquez
- Dave Fennoy as Howard
- Anthea Neri Best as Dr. Nguyen
- Natalia Del Riego as Odyssey
- Evan Michael Lee as Eugene
- Jake Green as Darren Shipman
Trivia
- Many of the suit styles were references to other costumes.
- Style 1 of Peter's Advanced Suit 2.0 had the color scheme of the Earth-51838 Peter Parker's advanced suit.
- Style 1 of the Black Suit and Style 2 of the Symbiote Suit were both based on the color scheme of Randy Schueller's original design for Spider-Man's Black Suit.
- Style 1 of Peter's Classic Suit matched that of Web-Man.
- Style 1 of Peter's Spider-Man 2099 Black Suit had the suit's original color scheme: black and red.
- Style 3 of Peter's Superior Suit was based on Superior Octopus' Suit.
- Style 3 of Peter's Iron Spider Armor had the black and gold look the armor had during Sinister Six Reborn.
- Style 2 of Peter's New Blue Suit resembled the Spider-Man of Earth-808122's Fantastic Four uniform.
- Style 1 of Classic Black Suit had the purple tint of the Venom Symbiote of Earth-1610, while style 3 was shaded blue and red like the Venom Symbiote of Earth-92131 had when bonded to Eddie Brock.
- Style 4 of the Last Stand Suit had the color scheme of Arachno-Man.
- Style 3 of Miles' Family Business Suit resembled the design of the Iron Spider Armor during Carnage Reigns.
- Style 3 of Miles' Sportswear Suit had the color scheme of the Earth-1610B Miles Morales' suit in Spider-Man: Into the Spider-Verse.
- Miles' Forever Suit was based on the Panther Habit of Earth-199999. Style 1 had the color scheme from Captain America: Civil War, Style 2 had gold highlights, like N'Jadaka and Shuri's suits, while style 3 had purple highlighhts, like T'Challa's suit.
- Miles' Best There Is Suit had the style of Wolverine's other suits. In order: classic brown and gold, X-Force, and classic yellow and blue.