Volume 8 Issue 6, June 2024

Photoacoustic tomography can image fast haemodynamics by either exploiting the spatial heterogeneity of blood or by leveraging a single laser pulse and a single element functioning as thousands of virtual detectors.

Fluorescence guidance is utilized to increase the chances of complete tumour resection while balancing preservation of neurological function in glioma surgery. A multimodal optical microscope capable of imaging the histology and fluorescence of fresh human brain specimens revealed an unexpected pattern of fluorophore accumulation and a new means of visualizing macrophages during surgery.

The clinical detection of fluorescent protoporphyrin IX during glioma-resection surgery can be improved via a microscope that pairs stimulated Raman histology and two-photon excitation fluorescence microscopy.

Transthoracic ultrasound localization microscopy enables super-resolution imaging of myocardial microvasculature and haemodynamics in patients with impaired myocardial function using data acquired within a breath hold.

Successive single-shot wide-field photoacoustic images can be used to estimate the speed and direction of blood flow at more than 5 mm deep from the skin’s surface.

Photoacoustic tomography using a single laser pulse and a single element functioning as thousands of virtual detectors allows for the volumetric capture of fast haemodynamic changes in the feet of human volunteers.

Multiplexed imaging of three types of infrared-light-emitting nanoparticle allows for the tracking of a nanoparticle-based cancer vaccine and of the elicited responses from intratumoural antigen-specific T lymphocytes in mice.

A wide-field fluorescence microscope leveraging a spinning disc and high-speed cameras enables the recording of neural activities and neutrophil trajectories at micrometric resolution on curved cortical surfaces in live mice.

An optimized head-mounted fluorescent mesoscope enables large-scale calcium imaging at single-neuron resolution in freely moving mice, facilitating neurobehavioural studies during social interactions and fear-conditioning experiments.

Bioluminescent sources can be detected with magnetic resonance imaging by leveraging the light-mediated activation of vascular cells expressing a photosensitive bacterial enzyme that causes alterations in local haemodynamic contrast.

Hydronium ions bordering cancer cells are highly concentrated into a small extracellular region, and in tumour tissue such severely polarized acidity correlates with the expression of monocarboxylate transporters and with the exclusion of cytotoxic T cells.