Featured Characters:

• Spider-Man

Supporting Characters:

• 
  

Antagonists:

• Hobgoblin (Final appearance)
• Lizard (First appearance)
• Graviton (First appearance)
• Carnage (First appearance)
  • Carnage
• Mysterio (Final appearance)

Other Characters:

• Black Cat (First appearance)
• Captain America (First appearance)
• Daredevil (First appearance)
• Human Torch (First appearance)
• Wolverine (First appearance)

Races and Species:

• Humans

Locations:

• Earth-TRN098
  • United States of America
    • New York City, New York
      • City Streets
      • Lab
      • Sewers
      • Amusement Park
      • Empire State Building

Items:

• Spider-Man's Suit
• Web-Shooters
• Captain America's Uniform
• Captain America's Shield
• Daredevil's Suit
• Fantastic Four Uniforms
• Wolverine's Suit
• Goblin Armor
• Pumpkin Bombs
• Connors Formula antidote
• Mysterio Suit

Vehicles:

• Goblin Glider

Peter Parker sees a newspaper headline claiming that Spider-Man has gone bad. He sets out to uncover who has framed him and to clear his good name, along the way he encounters various superheroes who offer their support, confirming that they knew he could never become a villain.

• The game's box cover uses an image of Spider-Man from the cover of Amazing Spider-Man #362 while Carnage is from the first page of Amazing Spider-Man #361. Both images were originally drawn by Mark Bagley and are flipped for the box cover.
• The cutscene depicting Captain America, Wolverine, Human Torch, and Daredevil letting Spider-Man go after Mysterio is a lifted from the Spider-Man variant cover of Amazing Spider-Man Annual #21.^[1]