Featured Characters:

• Spider-Man (Peter Parker)
• Captain America (Steve Rogers)

Supporting Characters:

• 
  

Antagonists:

• Doctor Doom (Victor von Doom)
  • Electro (Max Dillon)
  • Hobgoblin (Roderick Kingsley)
  • Rhino (Aleksei Sytsevich)
  • Boomerang (Fred Myers)
  • Grey Gargoyle (Paul Duval)
  • Oddball (Elton Healey)
  • Batroc (Georges Batroc)
  • Machete (Ferdinand Lopez)
  • Zaran (Maximillian Zaran)
  • Ratan (Bud Cable)
  • Eduardo Lobo
  • Mysterio (Quentin Beck)

Other Characters:

• Hulk (Illusion)
• George H.W. Bush

Races and Species:

• Humans

Locations:

• Earth-616

Items:

• Spider-Man's Suit
• Web-Shooters
• Captain America's Uniform
• Captain America's Shield
• Doctor Doom's Armor
• Rhino Armor
• Mysterio Suit

Vehicles:

• 
  

Dr. Doom has stolen a U.S. nuclear missile and now threatens to use it against New York City, unless the U.S. agrees to become an official territory of Latveria. President George H.W. Bush calls upon Captain America and Spider-Man to foil the villain's plot. The duo travel to Latveria and then split-up: Spider-Man will track down and disarm the missile, while Captain America will defeat and capture Dr. Doom.

• Because this game wasn't given an exclusive reality number, it is presumed that the story of the game took place in Mainstream continuity.

• The game came with an exclusive pack-in comic laying out the backstory for the game.