Featured Characters:

• Spider-Man

Supporting Characters:

• Human Torch
• Iron Fist
• Speedball

Antagonists:

• Beetle
• Lizard
• Mysterio
• Alistaire Smythe
• Green Goblin
• Scorpion
• Venom
  • Venom Symbiote
• Doctor Octopus
• Carnage
  • Carnage Symbiote

Other Characters:

• Daily Bugle
  • J. Jonah Jameson

Races and Species:

• Humans
• Symbiotes

Locations:

• Earth-TRN102
  • United States of America
    • New York
      • New York City

Items:

• Spider-Man's Suit
• Web-Shooters
• Scorpion's Suit
• Beetle Armor
• Mysterio Suit
• Goblin Armor
• Pumpkin Bombs
• Doctor Octopus' Tentacles

Vehicles:

• The Amazing Spider-Man: Lethal Foes was an action game released only in Japan for the Super Famicom, otherwise known as the Super Nintendo.

• Every password is the surname of the previously defeated villain, with the exception of Alistair Smythe, who gets the latter part of his codename. In progression: Jenkins, Connors, Beck, Slayer, Osborn, Scorpion, Brock and Octavius.