Featured Characters:

• Spider-Man (Peter Parker)

Supporting Characters:

• 
  

Antagonists:

• Sinister Six
  • Dr. Octopus (Otto Octavius)
  • Electro (Maxwell Dillon)
  • Hobgoblin (Jason Macendale Jr.)
  • Mysterio (Quentin Beck)
  • Sandman (William Baker)
  • Vulture (Adrian Toomes)

Other Characters:

• 
  

Locations:

• Earth-Unknown
  • United States of America
    • New York City, New York

Items:

• Spider-Man's Suit
• Web-Shooters
• Doctor Octopus' Tentacles
• Goblin Armor
• Pumpkin Bombs
• Mysterio Suit
• Vulture's Wings

Vehicles:

• Goblin Glider

Doctor Octopus is plotting the crowning caper of his criminal career... to rule the world! He has reunited the Sinister Six to reach his goal, and it appears nothing stands in the way of the super villains, except Spider-Man.

• The game is based on the Return of the Sinister Six story line found in Issues 334-339 of Amazing Spider-Man.
• The Sinister Six seen reflecting out of Spider-Man's eyes on the main image were from the cover of Amazing Spider-Man #337 by Erik J. Larsen.