Featured Characters:

• Fantastic Four (Final appearance)
  • Mister Fantastic (Reed Richards) (Final appearance)
  • Invisible Woman (Susan Storm) (Final appearance)
  • Human Torch (Johnny Storm) (Final appearance)
  • Thing (Ben Grimm) (Final appearance)

Supporting Characters:

• Silver Surfer (Norrin Radd) (First appearance)

Antagonists:

• Galactus (Mentioned) (Appears in Nintendo DS version only)
• Doctor Doom (Victor von Doom) (Final appearance) (Death)
• Terrax the Tamer (Tyros) (First appearance; dies)
• Red Ghost (Ivan Kragoff)
• Super-Apes (First appearance)
  • Unnamed apes (First appearance)
• Super-Skrull (Kl'rt) (First appearance)

Other Characters:

• T. Hager (First appearance; dies)

Races and Species:

• Humans (Final appearance)
• Zenn-Lavians (First appearance)
• Taa-ans (First appearance)
• Landlaks (First appearance)
• Skrulls (First appearance)

Locations:

• Earth-TRN1004 (Final appearance)
  • Earth (Final appearance)
    • United States of America (Final appearance)
      • New York (Final appearance)
        • New York City (Final appearance)
          • Manhattan (Final appearance)
            • Baxter Building (Final appearance)

Items:

• Silver Surfer's Surfboard (First appearance)

Vehicles:

• Fantasti-Car (First appearance)