Featured Characters:

• Blade (Eric Brooks) (First appearance)

Supporting Characters:

• Nightstalkers (First appearance)
  • Hannibal King (First appearance)
  • Abigail Whistler (First appearance)

Antagonists:

• "Dracula" / "Drake" (First appearance; dies) (Impersonates Dr. Vance)
• Danica Talos (First appearance)

Other Characters:

• Dr. Vance (Mentioned)
• Police Chief Vreede (Mentioned)
• Abraham Whistler (Mentioned)

Races and Species:

• Humans (First appearance)
• Vampires (First appearance)
• Human-Vampire Hybrids (First appearance)

Locations:

• Earth-TRN1310 (First appearance)

• The game is based on the plot of Blade: Trinity, but it contradicts its events (the most notable difference is the ending where Blade becomes human after defeating Dracula), therefore it cannot take place in the same continuity as the films and previous Blade games.