Featured Characters:

• Black Widow (Natasha Romanova) (Last appearance)
• Punisher (Frank Castle) (Last appearance)

Supporting Characters:

• Avengers (First appearance)
  • Iron Man (Tony Stark) (Last appearance)
  • War Machine (Lt. Col. James Rhodes) (Last appearance)
  • Captain Marvel (Carol Danvers) (First appearance)
  • Thor (First appearance)
  • Hulk (Bruce Banner) (First appearance)
  • Hawkeye (Clint Barton) (Last appearance)
• S.H.I.E.L.D. (Last appearance)
  • Nick Fury (Last appearance)
  • Maria Hill (Last appearance)
  • Amadeus Cho (First appearance)

Antagonists:

• Leviathan (First appearance)
  • Orion (Viktor Uvarov) (First appearance; dies)
  • Cain (First appearance; dies)
  • Elihas Starr (First appearance; dies)
  • Bio-Soldiers (First appearance)
  • Baron Helmut Zemo (First appearance)
  • Griffin (John Horton) (First appearance)
  • Graviton (Franklin Hall) (First appearance)
  • Grim Reaper (Eric Williams) (First appearance)
  • Taskmaster (Tony Masters) (First appearance)
  • Count Nefaria (Luchino Nefaria) (First appearance)

Other Characters:

• Kirby (First appearance)
• Maria Castle (Only in flashback) (First appearance) (Deceased)
• Lisa Castle (Only in flashback) (First appearance) (Deceased)
• Frank Castle, Jr. (Only in flashback) (First appearance) (Deceased)
• Wasp (Janet Van Dyne) (Mentioned)
• Captain America (Steve Rogers) (Mentioned)

Locations:

• Earth-101001 (Last appearance)
  • United States of America (Last appearance)
    • Seattle (Last appearance)
    • Miami (Last appearance)
  • Russia (Last appearance)
    • Slorenia (Last appearance)
  • Pakistan (Mentioned)
    • Karachi (Mentioned)
  • Hong Kong (Last appearance)
  • Madripoor (Last appearance)

Items:

• Iron Man Armor (Last appearance)
• War Machine Armor (Last appearance)
• Hawkeye's Bow (Last appearance)
• Trick Arrows (Last appearance)
• Black Widow's Gauntlets (Last appearance)
• Mjolnir (First appearance)
• Grim Reaper's Scythe (First appearance)

Vehicles:

• S.H.I.E.L.D. Helicarrier (Last appearance)
  • S.H.I.E.L.D. Interceptors (Last appearance)

• Jennifer Carpenter as Black Widow / Natasha Romanova
• Brian Bloom as Punisher / Frank Castle
• John Eric Bentley as Director Nick Fury
• Grant George as Elihas Starr
• Kyle Hebert as Cain
• Kari Wahlgren as Agent Maria Hill
• Eric Bauza as Amadeus Cho
• JB Blanc as Orion / Viktor Uvarov
• Fred Tatasciore as Hulk / Bruce Banner
• Matthew Mercer as Iron Man / Tony Stark