Membrane ATPases and acid tolerance of Actinomyces viscosus and Lactobacillus casei
- PMID: 2445289
- PMCID: PMC204068
- DOI: 10.1128/aem.53.9.2124-2128.1987
Membrane ATPases and acid tolerance of Actinomyces viscosus and Lactobacillus casei
Abstract
Lactobacillus casei ATCC 4646 and Actinomyces viscosus OMZ105E were found to differ markedly in acid tolerance. For example, pH profiles for glycolysis of intact cells in dense suspensions indicated that glycolysis by L. casei had an optimal pH of about 6.0 and that glucose degradation was reduced by 50% at a pH of 4.2. Comparable values for A. viscosus cells were at pHs of about 7.0 and 5.6. The difference in acid tolerance appeared to depend mainly on membrane physiology, and the addition of 40 microM gramicidin to cell suspensions increased the sensitivity of the glycolytic system by as much as 1.5 pH units for L. casei and up to 0.5 pH unit for A. viscosus. L. casei cells were inherently somewhat more resistant to severe acid damage than were A. viscosus cells, in that Mg release from L. casei cells in medium with a pH of 3.0 occurred only after a lag of some 4 h, compared with rapid release from A. viscosus cells. However, the major differences pertinent to the physiology of the organisms appeared to be related to proton-translocating ATPases. Isolated membranes of L. casei had about 3.29 U of ATPase per mg of protein, compared with only about 0.06 U per mg of protein for those of A. viscosus. Moreover, the ATPase of L. casei had a pH optimum for hydrolytic activity of about 5, compared with an optimal pH of about 7 for that of A. viscosus.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci.Infect Immun. 1986 Aug;53(2):331-8. doi: 10.1128/iai.53.2.331-338.1986. Infect Immun. 1986. PMID: 3015800 Free PMC article.
-
Anaerobic killing of oral streptococci by reduced, transition metal cations.Appl Environ Microbiol. 1998 Jan;64(1):27-33. doi: 10.1128/AEM.64.1.27-33.1998. Appl Environ Microbiol. 1998. PMID: 9435058 Free PMC article.
-
Fluoride inhibition of proton-translocating ATPases of oral bacteria.Infect Immun. 1987 Nov;55(11):2597-603. doi: 10.1128/iai.55.11.2597-2603.1987. Infect Immun. 1987. PMID: 2889674 Free PMC article.
-
[Synthesis of ATP by membrane-bound and soluble H+-ATPase from Lactobacillus casei during an abrupt increase in the medium pH].Dokl Akad Nauk SSSR. 1986;288(6):1494-6. Dokl Akad Nauk SSSR. 1986. PMID: 2873978 Russian. No abstract available.
-
Lactate dehydrogenase from Streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from Lactobacillus casei, Actinomyces viscosus, and Streptococcus sanguis.Infect Immun. 1985 Feb;47(2):489-95. doi: 10.1128/iai.47.2.489-495.1985. Infect Immun. 1985. PMID: 3917978 Free PMC article.
Cited by
-
Insight into the acid tolerance mechanism of Acetilactobacillus jinshanensis subsp. aerogenes Z-1.Front Microbiol. 2023 Jul 14;14:1226031. doi: 10.3389/fmicb.2023.1226031. eCollection 2023. Front Microbiol. 2023. PMID: 37520381 Free PMC article.
-
10-undecynoic acid is a new anti-adherent agent killing biofilm of oral Streptococcus spp.PLoS One. 2019 Apr 18;14(4):e0214763. doi: 10.1371/journal.pone.0214763. eCollection 2019. PLoS One. 2019. PMID: 30998699 Free PMC article.
-
The Lactobacillus casei Group: History and Health Related Applications.Front Microbiol. 2018 Sep 10;9:2107. doi: 10.3389/fmicb.2018.02107. eCollection 2018. Front Microbiol. 2018. PMID: 30298055 Free PMC article. Review.
-
A Comparison of the ATP Generating Pathways Used by S. Typhimurium to Fuel Replication within Human and Murine Macrophage and Epithelial Cell Lines.PLoS One. 2016 Mar 1;11(3):e0150687. doi: 10.1371/journal.pone.0150687. eCollection 2016. PLoS One. 2016. PMID: 26930214 Free PMC article.
-
Meta-omics uncover temporal regulation of pathways across oral microbiome genera during in vitro sugar metabolism.ISME J. 2015 Dec;9(12):2605-19. doi: 10.1038/ismej.2015.72. Epub 2015 May 29. ISME J. 2015. PMID: 26023872 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources